| （1.华南师范大学 体育科学学院，广东 广州 510006；2.临沂大学 体育学院，山东 临沂 276005） 摘 要：研究6周递增负荷运动对小肠集合淋巴结(PP结)线粒体膜电位(ΔΨm)、BaxmRNA、Bcl-2mRNA和细胞色素C(Cyt C)的影响，探讨PP结淋巴细胞线粒体凋亡途径指标的变化。将64只8周龄雄性SD大鼠随机分为运动组和对照组，运动组进行6周的递增负荷跑台训练，分别于第0、2、4、6周末，利用FQ-RT-PCR技术测定Bax和Bcl-2mRNA的表达水平；利用JC-1染色流式细胞术检测PP结淋巴细胞的平均J-aggregate( )和J-monomer( )的含量，并计算ΔΨm( / )；用ELISA法测定Cyt C的浓度。结果显示：(1)6周递增负荷运动过程中，在第2、4、6周，PP结淋巴细胞的ΔΨm显著低于0周，第4周的ΔΨm最低；(2)与0周相比，第4、6周PP结BaxmRNA表达明显增加，Bcl-2mRNA表达降低，Bcl-2/Bax值明显降低；(3)与0周相比，第2、4、6周PP结Cyt C浓度明显增加。结果表明递增负荷运动可能是通过影响线粒体途径从而影响PP结淋巴细胞凋亡的。 |
WANG Xue-qin1，2，HAO Xuan-ming1
| （1.School of Physical Education，South China Normal University，Guangzhou 510006，China；2.School of Physical Education，Linyi University，Linyi 276005，China） Abstract: The authors studied the effects of 6-week load gradually increased exercising on the membrane potential (ΔΨm), BaxmRNA, Bcl-2mRNA and Cytochrome C (Cyt C) of mitochondria in Peyer’s patches, and probed into the changing of such an index as mitochondrial apoptosis pathway in Peyer’s patches. The authors divided 64 8-week old male SD rats randomly into an exercising group and a control group, let the rats in the exercising group do a load gradually in-creased exercise on a treadmill for 6 weeks, measured the levels of Bax and Bcl-2mRNA expression by utilizing the FQ-RT-PCR technology and the average contents of J-aggregate ( ) and J-monomer ( ) of lymphocyte in Peyer’s patches by utilizing JC-1 staining flow cytometry at the end of weeks 0, 2 4 and 6 respectively, calculated ΔΨm ( / ), and measured the concentration of Cyt C by apply the ELISA method. The authors revealed the following findings: 1) during 6-week load gradually increased exercising, the ΔΨms of lymphocytes in Peyer’s patches calculated in weeks 2, 4 and 6 were significantly lower than that calculated in week 0, the ΔΨm calculated in week 4 was the lowest; 2) as compared with those measured in week 0, measured in weeks 4 and 6, the levels of BaxmRNA expression in Peyer’s patches increased significantly, the levels of Bcl-2mRNA expression decreased, and the ratio of Bcl-2 to Bax decreased significantly; 3) as compared with those measured in week 0, the concentrations of Cyt C in Peyer’s patches measured in weeks 2, 4 and 6 increased significantly. The said findings indicated that load gradually increased exercising might affect the apoptosis of lymphocytes in Peyer’s patches by affecting the mitochondrial pathway. |
下载本期全文：点击下载