| （1.商丘师范学院 体育学院，河南 商丘 476000；2.苏州大学 体育学院，江苏 苏州 215021） 摘 要：通过观察下坡跑运动后α7β1整合素及FAK的表达和FAK活性的变化，探讨α7β1整合素在运动重复效应中作用机制。将32只小鼠随机分为安静对照组，运动后3 h组、24 h组、8 d组。小鼠进行一次性下坡跑运动，速度为17 m/min，坡度为－20°，时间为30 min。各组于各时刻点摘眼球取血及取双侧腓肠肌。比色法检测血清CK活性，Real-time PCR检测α7β1整合素及FAK的mRNA表达，Western blot检测α7β1整合素及pFAK397的蛋白表达。结果显示一次下坡跑运动后CK活性升高显著，且在24 h增幅增大。α7 integrin mRNA在一次运动后3 h显著增加，但在24 h变化不明显，但8 d时仍增加显著，其蛋白水平在运动后3 h显著增加，虽在24 h和8 d时升高程度逐渐降低但仍呈显著。β1 integrin mRNA在一次运动后3 h显著增加，24 h及8 d变化不明显，蛋白水平在一次运动后3 h呈显著升高，24 h升高程度增加，在8 d时回复。FAK mRNA在一次运动后3 h显著增加，24 h及8 d变化不明显，pFAK397水平在一次运动后呈现显著升高，24 h升高程度降低，8 d时基本回复。结果表明α7β1整合素-FAK信号通路介导了运动重复效应。 |
LI Min1，ZHANG Lin2
| （1.School of Physical Education，Shangqiu Normal College，Shangqiu 476000，China；2.School of Physical Education，Soochow University，Suzhou 215021，China) Abstract: In order to probed into the mechanism of α7β1 integrin working on the repeated bout effect by observing the changing of the expression of α7β1 integrin and FAK as well as FAK activity after downhill running, the authors divided 32 mice randomly into a calm control group, a 3 h after running group, a 24 h after running group, and an 8d after running group, let the mice carry out one-time downhill running at a speed of 17 m/min, on a slope of -20°, for 30 minutes, took the eyeball blood and both-sides gastrocnemius of the mice in various groups at the said times, measured serum CK activity by means of colorimetry, measured the mRNA expression of α7β1 integrin and FAK by means of real-time PCR, measured the protein expression of α7β1 integrin and pFAK397 by means of Western blot, and revealed the following findings: after one-time downhill running, CK activity increased significantly, and the increment increased at 24 h; α7 integrin mRNA increased significantly at 3 h after one-time running, but changed insignificantly at 24 h, yet still increased significantly on day 8, its protein level increased significantly at 3 h after running, the degree of increasing was still significantly although it decreased gradually at 24 h and on day 8; β1 in-tegrin mRNA increased significantly at 3 h after one-time running, changed insignificantly at 24 h and on day 8, its protein level increased significantly at 3 h after one-time running, the degree of increasing increased at 24 h, but dropped back on day 8; FAK mRNA increased significantly at 3 h after one-time running, changed insignificantly at 24 h and on day 8, pFAK397 level increased significantly after one-time running, the degree of increasing decreased at 24 h, basically dropped back on day 8. The said findings indicate that α7β1 integrin and FAK signal pathway me-diated the repeated bout effect. |
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