| （惠州学院 体育系，广东 惠州 516007） 摘 要：研究运动对RBP4诱导的脂代谢异常鼠肝脏SREBP-1c信号通路和脂肪合成的影响；重组人RBP4注射建立脂代谢异常鼠模型，设一次性运动组(RBP4 One-time exercise group，ROE组)、8周有氧运动组(RBP4 aerobic exercise group，RAE组)和安静对照组(RBP4 control group，RC组)。结果发现：ROE组血清RBP4水平与RC组比较非常显著降低(P<0.01)，血清TG和肝脏TG含量无显著性改变(P>0.05)。RAE组血清RBP4、TG和肝脏TG含量与RC组和ROE组比较均非常显著降低(P<0.01)。与RC组比较，ROE组和RAE组肝脏SREBP-1c mRNA表达均非常显著降低(P<0.01)；ROE组SREBP-1c蛋白表达、FASmRNA和FAS蛋白表达均无显著性改变(P>0.05)；RAE组SREBP-1c、FAS的mRNA和蛋白表达比ROE组均非常显著减少(P<0.01)。结果说明RBP4显著使小鼠肝脏SREBP-1c基因和蛋白表达增加、激活SREBP-1c信号通路、促进肝脏脂肪合成。8周有氧运动抑制了RBP4在肝脏的脂肪合成促进作用，改善脂代谢，机制涉及降低RBP4水平，减少肝脏SREBP-1c基因和蛋白表达，抑制SREBP-1c信号通路的激活，降低肝脏脂肪合成功能。 |
ZHANG Ming-jun
| （Department of Physical Education，Huizhou University，Huizhou 516007，China) Abstract: In order to study the effects of exercise on SREBP-1c signal pathway and fat synthesis of the liver of mice with dyslipidemia induced by RBP4, the author established a dyslipidemia mouse model by means of RBP4 intraperitoneal injection, set up a one-time exercise group (ROE), an 8-week aerobic exercise group (RAE) and a calm control group (RC), and revealed the following findings: comparing the mice in group ROE with the mice in group RC, serum RBP4 level decreased significantly (P<0.01), serum TG and liver TG contents had no significant change (P>0.05); comparing the mice in group RAE with the mice in groups RC and ROE, serum RBP4, serum TG and liver TG contents decreased significantly (P<0.01); comparing the mice in groups ROE and RAE with the mice in group RC, liver SREBP-1c mRNA expression decreased significantly (P<0.01); as for the mice in group ROE, SREBP-1c protein expression, FAS mRNA and FAS protein expression had no significant change (P>0.05); com-paring the mice in group RAE with the mice in group ROE, SREBP-1c, FAS mRNA and protein expression de-creased significantly (P<0.01). The said findings indicate the followings: RBP4 significantly increased mouse liver’s SREBP-1c gene and protein expressions, activated SREBP-1c signal pathway, and promoted liver fat synthe-sis; 8-week aerobic exercise restrained the role of RBP4 in promoting fat synthesis in the liver, improved fat me-tabolism; the mechanism involved with reducing RBP4 level, reducing liver SREBP-1c gene and protein expres-sions, restraining SREBP-1c signal pathway activation, and reducing the liver’s fat synthesis function. |
下载本期全文：点击下载