| （华南师范大学 体育学院，广东 广州 510006） 摘 要：观察6周递增负荷运动中，大鼠小肠集合淋巴结(PP结)形态结构及淋巴细胞凋亡的基本特征。雄性SD大鼠64只，运动组进行6周递增负荷跑台运动，分别在第0、2、4、6周末观察小肠PP结个数和PP结形态学变化，并通过淋巴细胞Bax、Bcl-2表达和SOD、MDA水平探讨对淋巴细胞凋亡的影响。结果表明：1)PP结数目呈现先下降后回升的变化趋势。2)PP结结构改变主要发生在生发中心及拱顶区(B cell区)，第2~4周PP结生发中心出现断裂带，拱顶顶部出现空泡，第6周有所恢复。3)Bax与Bcl-2比值升高，提示淋巴细胞凋亡增多，且主要发生在PP结生发中心。4)运动应激性自由基生成可能是肠道淋巴结细胞凋亡的机制之一。 |
QIN Fei，HAO Xuan-ming
| （School of Physical Education，South China Normal University，Guangzhou 510006，China） Abstract: In order to observe the basic characteristics of the morphological structure and lymphocyte apoptosis of Peyer’s patches of rats during 6-week exercising with an increasing load, the authors divided 64 male SD rats into an exercise group and a control group, put the rats in the exercise group on a treadmill for 6-week exercising with an increasing load, observed the change of the number of Peyer’s patches and the morphological change of Peyer’s patches at the end of weeks 0, 2, 4 and 6 respectively, and probed into the effects of the expressions of lymphocytes Bax and Bcl-2 and the levels of SOD and MDA on lymphocyte apoptosis. The authors revealed the following find-ings: 1) the number of Peyer’s patches showed a trend of decreasing followed by increasing; 2) the change of the structure of Peyer’s patches mainly occurred in the germinal center and the dome zone (B cell zone); in weeks 2~4, rupturing belts appeared in the germinal center of Peyer’s patches, bubbles appeared at the top of the dome, the con-ditions were somewhat restored in week 6; 3) the ratio of Bax to Bcl-2 increased, which suggested that lymphocyte apoptosis increased, and mainly occurred in the germinal center of Peyer’s patches; 4) the generation of free radicals induced by exercising may be one of the mechanisms of intestinal lymphocyte apoptosis. |
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