| （华南师范大学 体育科学学院，广东 广州 510006） 摘 要：为了探讨有氧运动和白藜芦醇对肥胖大鼠脂联素受体及下游信号通路的影响及机制，将40只大鼠分成5组，分别为普通对照组(C)、肥胖模型对照组(D)、肥胖运动组(E)、白藜芦醇对照组(F)、白藜芦醇运动组(G)；造模完成后，E和G组采用6周跑台训练，F和G组按照大鼠称重后40 mg/kg的白藜芦醇(Res)剂量进行6周灌胃。用ELISA法测得血清脂联素；RT-PCR方法测得AdipoR1、AdipoR2及PPARγ、AMPKα蛋白表达。结果发现：(1)血清脂联素水平模型组低于普通对照组，而模型组中白藜芦醇运动组最高；(2)脂联素受体1和2的蛋白表达，白藜芦醇运动组均高于其它各模型组；(3)普通对照组PPARγmRNA表达最高，而AMPKαmRNA表达白藜芦醇运动组最高；以上结果说明，本实验中通过运动和白藜芦醇联合干预，提高血清脂联素及受体的水平，从而降低TG、GLU等相关指标，脂联素受体激活下游信号通路蛋白AMPKαmRNA和PPARγmRNA，并使其表达增加，从而在一定程度上改善大鼠内脏脂肪细胞的紊乱，减少脂肪合成，增加血清TG清除，减少脂肪组织脂肪积聚，对改善大鼠肥胖起到了一定的作用。 |
RUAN Ling，XIAO Guo-qiang
| （School of Physical Education，South China Normal University，Guangzhou 510006，China） Abstract: In order to probe into the effects of aerobic exercising and resveratrol on the adiponectin receptors and downstream signaling pathways of obese rats as well as the affecting mechanism, the authors divided 40 rats into 5 groups, which were respectively normal control group (C), obese model control group (D), obese exercising group (E), resveratrol control group (F), resveratrol exercising group (G); after model establishment was completed, the rats in groups E and G had 6-week treadmill training, while the rats in groups F and G were fed with resveratrol by gavage for 6 weeks at the dose of 40mg/kg after they were weighed. The authors measured serum adiponectin by using the ELISA method, measured AdipoR1, AdipoR2 and PPARγ and AMPKα protein expression by using the RT-PCR method, and revealed the following findings: 1) the levels of serum adiponectin of the rats in the model groups was lower than those of the rats in the normal control group, while the rats in the resveratrol exercising group in the model groups had the highest level of serum adiponectin; 2) the levels of protein expression of adi-ponectin receptors 1 and 2 of the rats in the resveratrol exercising group were all higher than those of the rats in other model groups; 3) the rats in the normal control group had the highest level of PPARγmRNA expression, while the rats in the resveratrol exercising group had the highest level of AMPKαmRNA expression. The said findings in-dicated the followings: In this experiment, the exercising and resveratrol combined intervention boosted the levels of serum adiponectin and receptors, thus lowering relevant indexes such as TG and GLU; adiponectin receptors ac-tivated downstream signaling pathway proteins AMPKαmRNA and PPARγmRNA, and increased their expression, thus improving the disordering of visceral fat cells of the rats, reducing fat synthesis, boosting the removal of serum TG and reducing fat tissues and fat accumulation to a certain extent, playing a certain role in improving rat obesity. |
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