| （华南师范大学，广东 广州 510006） 摘 要：通过胸腺细胞因子IL-7和TGF-β1及其mRNA进行研究，探讨运动性免疫抑制发生发展过程中胸腺细胞发育的调节机制。将128只8周龄雄性SD大鼠随机分为运动组和对照组，运动组进行递增负荷跑台训练6周，每周6次，周日休息，每次30 min。第1周负荷为10 m•min-1，第2周负荷为20 m•min-1，此后每周增加5 m•min-1，至6周时达40 m•min-1。分别于第0、2、4、6周利用ELISA和FQ-RT-PCR技术测相对安静状态、运动后即刻和运动后3 h IL-7和TGF-β1及其mRNA的表达水平。结果显示：6周递增负荷跑台运动过程中，IL-7和TGF-β1呈现几乎相反的应答性变化：IL-7及mRNA第0周、第2周末运动后明显降低，恢复3 h后升高，呈“V”型应答曲线；第4周末，运动前后没有显著性变化；第6周末，在运动后持续下降。TGF-β1在各周呈现倒“V”型变化，TGF-β1 mRNA对负荷初次应答时运动前后没有明显变化，其它各周呈倒“V”型变化。以上结果说明运动性免疫抑制发生发展中胸腺IL-7下降和TGF-β1升高可能导致胸腺微环境紊乱，从而影响胸腺细胞发育。 |
ZHANG Lin，HAO Xuan-ming
| （South China Normal University，Guangzhou 510006，China） Abstract: To investigate the regulatory mechanism of thymocyte development during exercise-induced immuno-suppression, IL-7 and TGF-β1 as well as their mRNA of rats in thymus were tested. A total of 128 Sprague–Dawle male rats, aged 8 weeks, were divided into Control Group and Exercise Group. Incremental ergomitry Exercise pro-tocol (10 m•min-1 in week1, 20 min week2, 5 m•min-1 increased per week, successive 6 weeks, 6 d per week, 30 min per day). Samples were taken in week 0, week 2, week 4 and week 6 before exercise, just after exercise and 3 h after exercise, respectively. IL-7 and TGF-β1 as well as their mRNA of rats in thymus were calculated by ELISA and FQ-RT-PCR. Results:1) During six weeks incremental exercise: The responsive tendency of IL-7 and its mRNA presented the shape of “V” letter during week 0 and week2，while there was no distinguished changes during week4, and last decreased in week6. TGF-β1 and its mRNA presented that of inversed “V” letter during every week, except its mRNA was no prominent change in week0. Conclusion: The decrease of IL-7 and increase of TGF-β1 during Exercise-induced immunosuppression could be resulted the disorder of thymic microenvironment which effected thymocyte development. |
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