| （1.华南师范大学 激光运动医学实验室，广东 广州 510631；2.华中科技大学 生物医学光子所，湖北 武汉 430074） 摘 要：采用跑台下坡跑模型，研究了不同剂量的低强度氦氖激光对大鼠力竭运动后骨骼肌自由基、一氧化氮(NO)代谢的影响。72只SD大鼠随机分成安静对照组，运动对照组，运动加低、中、高剂量激光处理组。各运动组的大鼠均进行一次性跑台力竭运动，运动加激光组的大鼠在运动后于腓肠肌处接受氦氖激光照射，每日1次，各剂量组照射参数分别为12、28和43 J/cm2(20、46和71 mW/cm2，10 min)。运动后24和48 h进行腓肠肌取材，检测指标为肌肉超氧化物歧化酶(SOD)、丙二醛(MDA)、一氧化氮合酶(NOS)以及一氧化氮(NO)。结果发现，力竭运动后，运动对照组的MDA水平和NOS活性显著升高，而SOD活性和NO变化不明显。43 J/cm2的激光照射能显著提高力竭运动后的SOD活性、降低MDA水平以及显著提高NOS活性和NO水平，而12和28 J/cm2的激光照射则作用不明显。由此表明，低强度氦氖激光能够提高大鼠力竭运动后骨骼肌抗氧化能力、降低自由基水平，并能促进骨骼肌NO的合成，提高NO水平，其作用是剂量和强度依赖性的。 |
LIU Xiao-guang1，2，ZHOU Yong-jian1，XIA Yi- shan1，YUAN Jian-qin1，LIU Cheng-yi1，2
| （1.Lab of Laser Sports Medicine，South China Normal University，Guangzhou 510006，China；2. Institute of Biomedical Photonics，Huazhong University of Science and Technology，Wuhan 430074，China） Abstract: This study has investigated the effects of low-level He-Ne laser irradiation at different doses on the me-tabolism of free radicals and nitric oxide (NO) of rat skeletal muscle after exhaustive exercise by using the animal model of downhill running. Seventy-two Sprague-Dawley rats were randomly divided into five groups: sedentary control group, exercise control group，exercise + low-dose laser group, exercise + medium-dose laser group, and exercise + high-dose laser group. Each rat in the exercise control group and the three exercise plus laser groups per-formed a bout of exhaustive downhill running on treadmill. Three exercise plus laser groups received He-Ne laser irradiation after exercise at gastrocnemius muscles daily. The irradiation parameters for three different dose laser groups were 12, 28, and 43 J/cm2（20, 46, and 71 mW/cm2，10 min）, respectively. Gastrocnemius muscles were sampled at 24 and 48 h after exercise. Muscle superoxide dismutase (SOD), malondiadehyde (MDA), nitric oxide synthase (NOS) and NO were analyzed. The exercise control group exhibited significant elevations in muscle MDA level and NOS activity after exhaustive exercise. He-Ne laser irradiation at 43 J/cm2 significantly enhanced muscle SOD activity, NOS activity and NO level and significantly reduced muscle MDA level after exercise, whereas the effects of the irradiation at 12 or 28 J/cm2 were unmarked. In conclusion, low-level laser irradiation could enhance muscle anti-oxidative capacity and reduce free radicals level, and promote to synthesize NO and increase NO level in a dose/intensity-dependent manner. |
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