| （1.湘南学院 体育系，湖南 郴州 423000；2.长沙航空职业技术学院 体育部，湖南 长沙 410124；3.湖南师范大学 体育学院，湖南 长沙 410012） 摘 要：为了解补充不同浓度赖氨酸对力竭运动大鼠心、肝组织细胞凋亡的影响，观察其对心、肝组织细胞凋亡调控基因Bax和Bcl-2的变化，探讨赖氨酸对力竭运动大鼠机体的保护作用。将32只大鼠进行3 d的跑台运动适应性训练后，随机分成4组：安静对照组、运动组、低浓度给药组和高浓度给药组。力竭运动大鼠均采用Bedford所建立的运动负荷模型。实验结束用免疫组织化学法检测心、肝组织细胞凋亡调控基因Bax和Bcl-2的阳性物质平均光密度值、面积和含量。结果：与对照组比较，运动组和给药组心、肝组织细胞凋亡调控基因Bax光密度值差异具有显著性意义(P﹤0.05)，与运动组比较，差异也具有显著性(P﹤0.05)，不同浓度赖氨酸的作用相当，差异没有显著性；运动组与对照组比较，心、肝组织细胞凋亡调控基因Bcl-2光密度值差异具有显著性意义(P﹤0.05)，给药组与运动组比较，心组织细胞凋亡调控基因Bcl-2光密度值差异也具有显著性意义(P﹤0.05)，只肝细胞表现高浓度给药组比低浓度给药组相比差异有显著性。结果说明：大强度急性力竭运动可使大鼠心、肝组织致损伤，诱导肝细胞凋亡，L-赖氨酸对急性力竭运动的大鼠心、肝组织有保护作用。 |
LUO Rong-bao1，ZHANG Gui-lan2，LIU Wen-feng3，LIU Hui-min3
| （1.Department of Physical Education，Xiangnan College，Chenzhou 423000，China；2.Department of Physical Education，Changsha Institute of Vocational and Technical Aviation，Changsha 400124，China；3.School of Physical Education，Hunan Normal University，Changsha 410012，China） Abstract: In order to gain an insight into the effects of lysine with different concentrations on the apoptosis of car-diac and hepatic histiocytes of rats doing an exhaustive exercise, to observe the change which lysine causes to Bax and Bcl-2 (genes that regulate the apoptosis of cardiac and hepatic histiocytes), and to probe into the function of ly-sine in protecting the body of rats doing an exhaustive exercise, the authors divided 32 rats randomly into 4 groups (calm control group, exercise group, low concentration lysine supplied group and high concentration lysine supplied group) for 3 days of adaptation training on a running track, adopted the exercise load model established by Bedford for rats doing an exhaustive exercise, used an immunohistochemical method to test the average optical density, area and content of the positive substance of Bax and Bcl-2 when the experiment was finished, and revealed the follow-ing findings: there is a significant difference (P<0.05) in optical density of Bax between the exercise group/lysine supplied groups and the control group, and also between the lysine supplied groups and the exercise group; no sig-nificant difference was produced by the function of lysine with different concentrations, whose function is pretty much the same; there is a significant difference (P<0.05) in optical density of Bcl-2 between the exercise group and the control group, and also between the lysine supplied groups and the exercise group, and there is a significant dif-ference in hepatic cell expression between the high concentration lysine supplied group and the low concentration lysine supplied group. From the said findings the authors drew the following conclusions: a high intensity acute ex-haustive exercise can cause the damage of cardiac and hepatic tissue of rats, and induce the apoptosis of hepatic cells; L-lysine has the function of protecting cardiac and hepatic tissue of rats doing an exhaustive exercise. |
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