| （1.暨南大学 体育学院，广东 广州 510032；2.国家体育总局体育科学研究所，北京 100061；3.华南师范大学 体育科学学院，广东 广州 510006） 摘 要：探讨低强度激光对运动性免疫抑制导致的肺组织炎症的调理作用。将8周龄雄性SD大鼠104只随机分为安静对照组、运动训练组、小剂量低强度激光+运动组、大剂量低强度激光+运动组。运动各组进行跑台训练，每次30 min，每天1次，每周6 d，持续6周。采用递增负荷强度(跑台速度第1周10 m/min、第2周20 m/min，从第3周开始，每周递增5 m/min，至第6周达到40 m/min)。激光干预采用氦氖激光鼻腔外照射(训练1次/d，2 min/次)。辐射功率小剂量组为1 mW (6.8 J/cm2)，大剂量组2 mW (13.6 J/cm2)。分别于训练前及训练的第2、4、6周周末取材，检测肺灌洗液中sIgA、CRP、IL-4、IL-10、TNF-α、ICAM-1含量；分析肺组织中CD4+和CD8+阳性蛋白表达，并对肺脏进行组织形态学观察。结果发现：1)6周递增负荷训练期间，大鼠肺脏中促炎因子(TNF-α和ICAM-1)显著上升，抗炎因子(IL-4和IL-10)及sIgA、CD4+与CD8+比值显著下降。肺组织局部出血、脓液渗出、炎性细胞浸润逐渐加重。2)小剂量低强度激光照射可显著延缓运动大鼠肺组织上述变化，对抗炎因子和形态学的影响尤为显著；而大剂量低强度激光的干预效果随照射疗程的延长呈下降趋势。结果说明：小剂量的低强度激光鼻部照射可显著促进6周递增负荷训练大鼠的免疫机能和肺组织炎症反应的改善，其调节过程主要是通过上调抗炎因子的合成，促使“促炎-抗炎”细胞因子平衡。 |
QIN Fei1，2，ZHAO Jie-xiu2，WANG Song-tao3，HAO Xuan-min3
| （1.School of Physical Education，Jinan University，Guangzhou 510032，China；2.China Institute of Sport Science，Beijing 100061，China；3.School of Physical Education，South China Normal University，Guangzhou 510006，China) Abstract: In order to probe into low-power laser’s function on regulating lung tissue inflammation caused by exer-cise-induced immunosuppression, the authors divided 104 8-week old male SD rats randomly into a calm control group, an exercise training group, a small-dose low-power laser + exercise group, and a big-dose low-power laser + exercise group. The rats in the exercise groups carried out treadmill training for 6 weeks continuously, 30min/time, 1 time/day, 6 days/week. The authors used incremental load intensities (treadmill speed: 10m/min in week 1, 20m/min in week 2, then increased incrementally by 5m/min per week, starting from week 3, to 40m/min in week 6). Laser intervention used was He-Ne laser nasal external irradiation (1 time/day on training days, 2min/time). The irradiation power was 1mW (6.8 J/cm2) for the small-dose group, 2mW (13.6 J/cm2) for the big-dose group. The authors took samples respectively before training and at the ends of weeks 2, 4 and 6, measured the contents of sIgA, CRP, IL-4, IL-10, TNF-α and ICAM-1 in the bronchoalveolar lavage fluid, analyzed the expressions of posi-tive proteins CD4+ and CD8+ in the lung tissues, and carried out histomorphological observation on the lungs, and revealed the following findings: 1) during the 6-week incremental load training period, in the rats’ lungs, pro-inflammation cytokines TNF-α and ICAM-1 increased significantly, anti-inflammation cytokines IL-4 and IL-10 as well as sIgA and the ratio of CD4+ to CD8+ decreased significantly; the lung tissues showed local breeding, pus exudation, and gradually worsening inflammatory cell infiltration; 2) small-dose low-power laser irradiation can significantly slow the said changes of the exercise rats’ lung tissues, its effects on anti-inflammation cytokines and morphology were especially significant; while the effect of big-dose low-power laser intervention showed a trend of decreasing as the irradiation process extended. The said findings indicate the followings: small-dose low-power la-ser nasal irradiation can significantly promote the improvement of immune function and lung tissue inflammation reaction of the rats carrying out 6-week incremental load training, its regulating process is mainly to promote the balance between pro-inflammation cytokines and anti-inflammation cytokines by up-regulating the synthesis of anti-inflammation cytokines. |
下载本期全文：点击下载