| （惠州学院 体育系，广东 惠州 516007） 摘 要：为了解运动对Fetuin-A诱导的胰岛素抵抗鼠骨骼肌IR和IRS1磷酸化的影响。运用牛fetuin-A 注射建立胰岛素抵抗鼠模型。将小鼠分为一次性运动组(Acute exercise group，AE)、8周运动组(Exercise group，E)、安静对照组(Control group，C)。结果：与C组比较，AE组和E组胰岛素抵抗指数均非常显著下降(P<0.01)；AE组骨骼肌IRS1(Tyr612)磷酸化非常显著增加(P<0.01)；E组骨骼肌IR(Tyr1150)磷酸化和IRS1(Tyr612)磷酸化均非常显著增加(P<0.01)；AE组和E组骨骼肌GLUT4mRNA表达和骨骼肌葡萄糖摄取率均非常显著增加(P<0.01)。结果说明：运动能够非常显著改善Fetuin-A诱导的胰岛素抵抗，8周有氧运动机制与增加骨骼肌IR(Tyr1150)、IRS1(Tyr612)磷酸化和GLUT4 mRNA表达有关。急性运动与增加骨骼肌IRS1(Tyr612)磷酸化和GLUT4 mRNA表达有关。 |
ZHANG Ming-jun
| （Department of Physical Education，Huizhou University，Huizhou 516007，China) Abstract: In order to understand the effects of exercising on IR and IRS1 phosphorylation of the skeletal muscle of Fetuin-A induced insulin resistance mice, the author established an insulin resistance mouse model by applying bo-vine Fetuin-A intraperitoneal injection, divided the mice into an acute exercising group (AE), an 8-week exercising group (E) and a calm control group (C). Results: as compared with the mice in group C, the insulin resistance in-dexes of the mice in groups AE and E decreased very significantly (P<0.01); IRS1 (Tyr612) phosphorylation of the skeletal muscle of the mice in group AE increased very significantly (P<0.01); IR (Tyr1150) and IRS1 (Tyr612) phosphorylation of the skeletal muscle of the mice in group E increased very significantly (P<0.01); the GLUT4mRNA expression and glucose intake rate of the skeletal muscle of the mice in groups AE and E increased very significantly (P<0.01). The results indicated the followings: exercising can improve Fetuin-A induced insulin resistance very significantly; the 8-week exercising mechanism is related to the increasing of IR (Tyr1150) and IRS1 (Tyr612) phosphorylation and GLUT4mRNA expression of skeletal muscle; acute exercising is related to the in-creasing of IRS1 (Tyr612) phosphorylation and GLUT4mRNA expression of skeletal muscle. |
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