| （华南师范大学 体育科学学院，广东 广州 510006） 摘 要：为探讨有氧运动和白藜芦醇(Res)对II型糖尿病(T2DM)大鼠肝脏氧化应激及NF-κBp65的影响，采用5周高糖高脂饮食加小剂量注射链脲佐菌素(STZ)(35 mg/kg)构建T2DM大鼠模型。将40只SPF级雄性SD大鼠随机分为5组，分别为正常对照组(C组)、T2DM模型组(D组)、“T2DM+运动”组(DE组)、“T2DM+Res”组(DR组)、“T2DM+运动+Res”组(DER组)，每组8只。运动为不负重游泳训练，每周6 d，Res采用灌胃方法按照45 mg/(kg•d)进行，每周7 d，7周后检测大鼠空腹血糖(FBG)、空腹血胰岛素(FINS)和肝脏TG、TC、SOD、MDA、TNF-a以及SREBP1、SREBP2和NF-κBp65核蛋白的表达。结果发现，1)与D组比较，DE组、DR组和DER组大鼠FINS、HOMA-IR均显著下降(P<0.05)，FBG呈降低趋势(P>0.05)，DER组与DE组、DR组比较，FBG、FINS和HOMA-IR均进一步降低(P>0.05)；2)与D组比较，DE组、DR组和DER组大鼠肝脏SOD活性显著升高(P<0.05)，而MDA含量，肝指数，肝脏TG、TC、nSREBP1、TNF-a、NF-κBp65核蛋白均显著性降低(P<0.05或P<0.01)，而nSREBP2呈降低趋势(P>0.05)，DER组较DE组、DR组比较，上述指标均进一步降低。结果说明，7周有氧运动和Res均能改善T2DM大鼠肝脏氧化应激和炎症反应，两者联合干预较单一效果更为显著，其机制可能通过抑制肝脏nSREBPs从而减少肝脏的脂质沉积引起的氧化应激，最终抑制NF-κBp65诱导的TNF-a的激活而实现。 |
CAO Jiao，XIAO Guo-qiang
| （School of Physical Education，South China Normal University，Guangzhou 510006，China) Abstract: In order to probe into the effects of aerobic exercise and resveratrol (Res) on oxidative stress and NF-κBp65 in the livers of type II diabetic (T2DM) rats, the authors established a T2DM rat model by feeding the rats with high sugar and high fat food and injecting a small dose (35 mg/kg) of streptozotocin (STZ) into the rats for 5 weeks, divided 40 class SPF male SD rats randomly into 5 groups, namely, a normal control group (group C), a T2DM group (group D), a “T2DM+exercise” group (group DE), a “T2DM+Res” group (group DR), and a “T2DM+exercise+Res” group (group DER), each of which contained 8 rats. The exercise was unloaded swimming training, done 6 d a week; the Res was orally administrated at a rate of 45 mg/(kg•d), 7 d a week. 7 weeks later, the authors measured the rats’ fasting blood glucose (FBG), fasting insulin (FINS), and liver TG, TC, SOD, MDA and TNF-a, and the expression of nucleo-proteins nSREBP1, nSREBP2 and NF-κBp65, and revealed the following findings: 1) comparing the rats in groups DE, DR and DER with the rats in group D, FINS and HOMA-IR decreased significantly (P<0.05), while FBG showed a trend of decreasing (P>0.05); comparing the rats in group DER with the rats in groups DE and DR, FBG, FINS and HOMA-IR decreased further (P>0.05); 2) comparing the rats in groups DE, DR and DER with the rats in group D, liver SOD activity increased significantly (P<0.05), while MDA content, liver index, nucleoproteins TG, TC, nSREBP1, TNF-a and NF-κBp65 in the livers decreased significantly (P<0.05 or P<0.01), while SREBP2 showed a trend of de-creasing (P>0.05); comparing the rats in group DER with the rats in groups DE and DR, all the said indexes decreased further. The said findings indicated the followings: both 7-week aerobic exercise and Res can improve oxidative stress and inflammatory reaction in the livers of T2DM rats; aerobic exercise and Res combined intervention produced an ef-fect more significant than the effect produced by aerobic exercise intervention or Res intervention alone, its mechanism may be realized by suppressing nSREBPs in the livers, thus reducing oxidative stress caused by lipid deposition in the livers, and ultimately suppressing the activation of TNF-a induced by NF-κBp65. |
下载本期全文：点击下载