| （华南师范大学 体育科学学院，广东 广州 510006） 摘 要：探讨高温预处理与不同强度的一次性急性运动共同干预下所诱导的热休克蛋白70，对大鼠海马区自由基以及钙ATP酶的影响。将3月龄SD大鼠48只，分为室温安静组、室温15 m/min运动组、室温27 m/min运动组，高温安静组、高温15 m/min运动组和高温27 m/min运动组等6组，每组8只。高温组大鼠经高温预处理24 h后，与其他运动组进行一次性急性运动60 min后即刻取材检测海马区热休克蛋白Hsp70、丙二醛(MDA)含量，超氧化物歧化酶SOD、Ca2+-ATPase活性。结果发现室温27 m/min运动组较之室温安静组，其大鼠海马区Hsp70的表达水平明显升高(P<0.05)；高温预处理各组的Hsp70表达水平，显著高于室温各组(P<0.05)；与室温运动组比较，高温预处理运动组海马SOD的活性提高极明显(P<0.01)；各高温组MDA含量随运动强度增大而降低，但组间差异无显著性(P>0.05)；高温预处理各组Ca2+-ATPase活性均显著高于室温各组(P<0.05)。结果说明：高温预处理诱导Hsp70的大量表达可提高运动时海马的SOD活性，降低MDA含量，提高Ca2+-ATPase活性。提示Hsp70可能通过提高抗氧化能力，以减少自由基对海马区的损害，对神经系统具有一定的保护意义。 |
LI Han，XIAO Guo-qiang，ZHU Shi
| （School of Physical Education，South China Normal University，Guangzhou 510006，China） Abstract: In order to probe into the influence of heat shock protein 70 induced by the joint intervention of high temperature pretreatment and one-time acute exercising with different intensities on free radicals and Ca2+-ATPase in the hippocampus area of rats, the authors divided 48 SD 3-month old rats into 6 groups: a room temperature calm group, a room temperature 15m/min exercising group, a room temperature 27m/min exercising group, a high tem-perature calm group, a high temperature 15m/min exercising group, and a high temperature 27m/min exercising group. 24h after the rats in high temperature groups (8 in each group) were pretreated by high temperature, they did a 60min one-time acute exercise together with the rats in other exercising groups, and immediately after that, hot shock protein Hsp70 and MDA contents as well as SOD and Ca2+-ATPase activities in the hippocampus were sam-ple taken and tested. The results were as follows: comparing the rats in the room temperature 27m/min exercising group with the rats in the room temperature calm group, the level of expression of Hsp70 in the hippocampus area rose significantly (P<0.05); the levels of expression of Hsp70 of the rats in groups pretreated by high temperature were significantly higher than those of the rats in room temperature groups (P<0.05); as compared with the rats in room temperature exercising groups, the SOD activity of hippocampus of the rates in exercising groups pretreated by high temperature increased extremely significantly (P<0.01); the MDA contents of the rats in high temperature groups decreased as exercise intensity increased, but showed no significant difference; the Ca2+-ATPase activities of the rats in groups pretreated by high temperature were all significantly higher than those of the rats in room tem-perature groups (P<0.05). The results indicated the followings: a lot of Hsp70 expression induced by high tempera-ture pretreatment can boost the SOD activity of hippocampus during exercising, lower MDA content, and boost Ca2+-ATPase activity. It is suggested that Hsp70 can reduce the damage to the hippocampus area cased by free radi-cals by enhancing antioxidation ability, thus it is provided with certain significance in protecting the nervous system. |
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