| （1.潍坊学院 体育学院，山东 潍坊 261061；2.辽宁省足球运动管理中心，辽宁 沈阳 110081；3.温州医学院 体育科学学院，浙江 温州 325035） 摘 要：为探讨急性力竭运动对青少年田径运动员血浆游离DNA(cf-DNA)的影响，寻找运动员机能监控的新标记物。安排16名青少年田径运动员在电动跑台上进行一次急性递增负荷力竭运动，于运动前、运动后即刻、运动后15 min、30 min、1 h、2 h、3 h、4 h、12 h和24 h分别测定红细胞压积(HCT)、cf-DNA、血浆丙二醛(MDA)、肌酸激酶(CK)和肌红蛋白(Mb)。用心率表监控运动过程并记录运动时间和心率，推算训练冲量(TRIMPS)。结果显示：HCT在所有监测时间点均无显著性变化(P>0.05)；cf-DNA在运动后即刻显著性升高(P<0.01)，30 min达峰值(P<0.01)，4 h后降至安静水平(P>0.05)；血浆MDA、CK和Mb在运动后即刻均显著性升高(P<0.05、P<0.01、P<0.01)，并一直持续到运动后24 h(P<0.01、P<0.05、P<0.01)。TRIMPS与运动后15 min和30 min时cf-DNA水平呈显著正相关(分别为r=0.55，P<0.01；r=0.64，P<0.05)。结果说明：cf-DNA是组织损伤早期标志物，可作为运动员机能监控的新指标。 |
WANG Yong1，FU Bo2，ZHU Rong3
| （1.School of Physical Education，Weifang University，Weifang 261061，China；2.Liaoning Center of Football Administration，Shenyang 110081，China；3.School of Physical Education，Wenzhou Medical College，Wenzhou 325035，China） Abstract: In order to probe into the effects of acute exhaustive exercising on cell-free plasma DNA of teenage track and field athletes, and to seek for a new marker for athlete performance monitoring, the authors arranged 16 teenage track and field athletes to do an acute exhaustive exercise on a power treadmill, tested their Hematocrit (HCT), Cf-DNA, plasma malondialdehyde (MDA), creatine kinase (CK) and myoglobin (Mb) respectively before exercis-ing, immediately after exercising, at 15 min, 30 min, 1 h, 2 h, 3 h, 4 h, 12 h and 24 h after exercising, monitored the exercising process with a heat rate meter, recorded their exercising times and heart rates, calculated their exercising impulses (TRIMPS), and revealed the following findings: there was no significant change to HCT at all test times (P>0.05); cf-DNA increased significantly immediately after exercising (P<0.01), reached the peak value at 30 min (P<0.01), and lowered to the calm level at 4 h (P>0.05); plasma MDA, CK and Mb increased significantly immedi-ately after exercising (P<0.05, P<0.01, P<0.01), and maintained up to 24 h after exercising (P<0.01, P<0.05, P<0.01); TRIMPS was positively correlative significantly with the cf-DNA level at 15 min and 30 min after exer-cising (r=0.55, P<0.01 and r=0.64, P<0.05 respectively). The said findings indicated that cf-DNA is an early marker for tissue damage, can be used as a new index for athlete performance monitoring. |
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