| （1.华南师范大学 体育科学学院，广东 广州 510006；2.怀化学院 体育系，湖南 怀化 418008） 摘 要：通过观察急性运动和热预处理后急性运动对大鼠股四头肌热休克蛋白(HSP)70表达影响的时相性变化和氧化应激标志物超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量的变化，探讨HSP70对急性运动大鼠股四头肌抗氧化能力的影响和股四头肌HSP70表达的应答性反应。雄性SD大鼠随机分为室温安静组、室温运动后即刻组、室温运动后3 h组、室温运动后24 h组、热预处理安静组、热预处理运动后即刻组、热预处理运动后3 h组、热预处理运动后24 h组。热预处理组在恒温干燥箱进行热暴露处理(直肠温度达到(41.5±0.5)℃，持续15 min)。安静组大鼠在热预处理24 h后对股四头肌进行取样。运动组大鼠在热预处理24 h后进行急性跑台运动(速度为25 m/min，坡度为0°，时间为60 min)，分别在运动后即刻、运动后3 h、运动后24 h对大鼠进行股四头肌取样，检测HSP70蛋白表达量、SOD活性和MDA含量。结果表明，大鼠急性运动后两组大鼠运动后即刻HSP70蛋白表达有上升的趋势，但差异无统计学意义(P>0.05)，3 h显著增加(P<0.05)，24 h后回降，室温组与基础水平相比差异无显著性(P>0.05)，而热预处理组仍显著高于基础水平(P<0.05)，在变化过程中热预处理组HSP70蛋白表达量显著高于室温组(P<0.05)；室温组和热预处理组大鼠急性运动后即刻股四头肌SOD活性明显升高(P<0.05)，3 h后活性降低，但显著高于基础水平(P<0.05)，24 h后回降到基础水平。在变化过程中热预处理组SOD活性显著高于室温组(P<0.05)；室温组和热预处理组大鼠运动后即刻股四头肌MDA的含量显著升高(P<0.05)，3 h后热预处理组MDA含量恢复至基础水平，室温组含量减少，但是仍显著高于基础水平(P<0.05)，室温组24 h恢复至基础水平，在变化过程中热预处理组较室温组MDA含量降低速率快(P<0.05)。这些发现表明急性运动可以诱导HSP70表达增加和热预处理后进行运动可以产生HSP70表达的累加效应。诱导HSP70大量表达可能提高机体的抗氧化能力，减轻机体运动时的氧化应激损伤。 |
WANG Jun-li1，2，XIAO Guo-qiang1
| （1.School of Physical Education，South China Normal University，Guangzhou 510006，China；2.Department of Physical Education，Huaihua University，Huaihua 418008，China） Abstract: The purpose of this study was to examine the effcet of acute exercise and acute exercise after hyperther-mic treatment on hsp70 and oxidative stress markers SOD and MDA At the same time, we investigated hsp70 re-sponse changes to exercise.Adult male SD rats were divided into eight groups: normothermic sedentary (Norm sed), normothermic exercise 0 h (Norm Exe 0 h), normothermic exercise 3 h(Norm Exe 3 h), normothermic exercise 24 h (Norm Exe 24 h), heat stress sedentary (Heat Sed), heat stress exercise 0 h (Heat Exe 0 h), heat stress exercise 3 h (Heat Exe 3 h), heat stress exercise 24 h (Heat Exe 24 h). The rats were heated until the rectal temperature reached (41.5±0.5) ℃ for 15 min in oven.Then the rats of Heat Sed were at rest under the normal temperature for 24 h.The rats were killed according to specified time after acute treadmill running (velocity is 25 m/min, gradient is 0°, time is 60 min) and quadriceps femories were sampled to analyze HSP70, SOD, MDA.The results were as follows: HSP70 content had no significant change immediately postexercise(P>0.05), HSP70 content increased significantly After 3 h (P<0.05), and it returned to the basal level at 24 h(P>0.05). The HSP70 content is higher in Heat Sed than Norm Sed during the process(P<0.05)；SOD activity in quadriceps femories elevated significantly immediately after an acute exercise in both Seds(P<0.05). Its activity decreased after 3 h(P<0.05) and returned the basal level at 24 h. The SOD activity is higher in Heat Sed than Norm Sed during the process(P<0.05)；(4)MDA content increased immediately postexercise(P<0.05). Its content decreased, but still higher than Norm Exe 0 h Sed, after 3 h(P<0.05). Its content renuned the basal level at 3 h in Heat sed.MDA content returned the basal level at 24 h in Nrom sed. Compared with Norm sed, the MDA is eliminated more easily in Heat Sed(P<0.05). These finds demonstrated acute ecercise induced the expression of HSP70 and acute exercise after hyperthermic treatment can produce a cumulative effect of HSP70 .HSP70 is expressed largely by hyperthermic treatment may increase antioxidant capacity and re-duce oxidative stress damage caused by the exercise. |
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