| （1.广州中医药大学 体育健康学院，广东 广州 510006；2.广州中医药大学 中药资源与工程研究中心，广东 广州 510006） 摘 要：采用中等强度持续性水平跑模型，研究了不同时相、不同剂量下抗氧化剂摄入大鼠骨骼肌细胞线粒体MDA、SOD、CAT、GSH-PX等抗氧化酶活性的变化，推测引发自由基稳态水平变化的先导性因子。90只SD大鼠，实验1随机分为正常人参皂甙Rb1组、7倍人参皂甙Rb1组、生理盐水模型组和生理盐水空白组，每组10只，检测指标为肌细胞线粒体SOD、MDA、CAT、GSH-PX；实验2随机分为空白组、中等强度即刻组、中等强度24 h组、中等强度48 h组、中等强度72 h组，每组10只，运动后即刻、24 h、48 h、72 h后取材，检测指标同实验1。结果发现：正常剂量人参皂甙组、7倍剂量人参皂甙组与生理盐水空白组相比抗氧化酶的活性显著增加。提示：运动应激过程中机体氧化能力的增高伴随着机体抗氧化能力的提高，但空白刺激条件下机体抗氧化能力的增强没有逆向引发自由基稳态系统反应。进一步推测：氧化能力是自由基稳态水平移动的先导因素，而外源性引发抗氧化能力提高对自由基积累无负反馈效应。 |
PAN Hua-shan1，TAN Jing2，LIU Gang1
| （1.School of Sport and Health，Guangzhou University of Chinese Medicine，Guangzhou 510006，China；2.Chinese Resources and Research Center,Guangzhou University of Chinese Medicine，Guangzhou 510006，China） Abstract: By using a continuous medium intensity horizontal running model, the authors studied the dynamic changes of the activity of anti-oxidation enzymes such as MDA, SOD, CAT and GSH-PX in the mitochondrion of skeletal muscle cells of rats taking in different dosages of antioxidant at different times, and inferred the lead factor that triggered the change of the level of stability of free radicals. The authors randomly divided 90 SD rats into a normal ginsenoside Rb1 group, a septuple ginsenoside Rb1 group, a physiological saline model group, and a physiological saline control group with 10 rats in each group in experiment 1, in which the test indexes were the SOD, MDA, CAT and GSH-PX in the mitochondrion of muscle cells, and into a control group, a medium intensity immediate group, a medium intensity 24h group, a medium intensity 48h group, and a medium intensity 72h group with 10 rats in each group in experiment 2. The author revealed the following finding: as compared with the physiological saline control group, the activity of antioxidant enzymes of rats in the normal ginsenoside group and the septuple ginsenoside group increased significantly, which suggested that during kinetic stressing the increase of the body’s anti-oxidation capacity was accompanied by the enhancement of the body’s anti-oxidation capacity, but the increase of the body’s anti-oxidation capacity under the stimulation free condition did not reversely trigger the reaction of the free radical stabilization system. The author further inferred that oxidation capacity is the lead factor for the horizontal movement of free radicals, while the enhancement of anti-oxidation capacity triggered exoge-nously has no negative feedback effect on the accumulation of free radicals. |
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