| （1.福建体育职业技术学院 运动人体科学教研室，福建 福州 350003；2.宁德师范高等专科学校 体育系，福建 宁德 352100） 摘 要：为了解不同负荷游泳运动对高脂血症大鼠血清CRP、IL-6以及TNF-α的影响，将雄性Wistar大鼠50只，随机抽取10只作为正常对照组(NC)，其余为高脂组。高脂血症模型建立成功后再将高脂组大鼠随机分成高脂血症安静对照组(HC)、高脂血症1 h有氧运动组(HE1)、高脂血症2 h有氧运动组(HE2)和高脂血症一次力竭运动组(HE3)。然后各组动物酶免法测定血清CRP，放免法测定血清IL-6、TNF-α。结果：(1)HC组大鼠血清CRP非常显著高于NC组(P＜0.01)，HE1组显著低于HC组(P＜0.05)，但仍明显高于NC组(P＜0.05)。(2)HC组大鼠血清IL-6显著高于NC组(P＜0.05)，HE3组非常显著高于HC组(P＜0.01)。(3)HC组大鼠血清TNF-α显著高于NC组(P＜0.05)，HE2组大鼠非常明显高于HC组(P＜0.01)。(4)CRP与TNF-α之间呈显著的低度负相关(r=-0.460，P=0.008)。结果表明，就循环CRP、IL-6、TNF-α水平而言，并非运动时间越长，对高脂血症越有好处，因此，不提倡一次长时间有氧运动，更不提倡达到疲劳状态；运动引起高脂血症大鼠血清CRP的变化可能与IL-6无关。 |
WANG Qing-bo1，LI Jin-hua2
| （1.Department of Humen Exercise Science，Fujian Sports Vocational Education and Technical College，Fuzhou 350003，China；2.Department of Physical Education，Ningde Teachers College，Ningde 352100，China） Abstract: In order to understand the effects of swimming under different loads on the CRP, IL-6 and TNF-α levels in blood serum of rats with hyperlipemia, the authors randomly selected 10 rats out of 50 male Wistar rats to form a normal control (NC) group and put the rest into a hyperlipemia group, randomly divided the rats in the hyperlipemia group into a hyperlipemia calm control (HC) group, a hyperlipemia with 1-hour aerobic exercise (HE1) group, a hyperlipemia with 2-hour aerobic exercise (HE2) group and a hyperlipemia with one time exhausted exercise (HE3) group after having successfully established a hyperlipemia model, and then measured the CRP level in blood serum of the rats in various groups by means of ELISA, as well as the IL-6 and TNF-α levels in blood serum of the rats in various groups by means of radioimmunoassay. Results: 1) the CRP level in blood serum of the rats in the HC group is significantly higher than the same of the rats in the NC group (P<0.01); the CRP level in blood serum of the rats in the HE1 group is significantly lower than the same of the rats in the HC group (P<0.05), but still higher than the same of the rats in the NC group (P<0.05); 2) the IL-6 level in blood serum of the rats in the HC group is signifi-cantly higher than the same of rats in the NC group (P<0.05); the IL-6 level in blood serum of the rats in the HE3 group is significantly higher than the same of rats in the HC group (P<0.01); 3) the TNF-α level in blood serum of the rats in the HC group is significantly higher than the same of the rats in the HC group (P<0.05); the TNF-α level in blood serum of the rats in the HE2 group is significantly higher than the same of the rats in the HC group (P<0.01); 4) there is a significant low degree negative correlation between the CRP and TNF-α levels: r =-0.460, P =0.008. The results reveal that in terms of circulative CRP, IL-6 and TNF-α levels, it is not true that the longer the exercise time, the better the hyperlipemia treatment; therefore, long time aerobic exercising at a time is not recom-mended, not to mention to do such an exercise long enough to reach the fatigue state; the change of the CRP level in blood serum of rates with hyperlipemia caused by exercising may be irrelevant to the IL-6 level. |
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